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CLEC16A Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Cow
  • Pig
Overview
Catalog # bs-18539R-PerCP-100uL
Product Name CLEC16A Polyclonal Antibody, PerCP Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Cow, Pig
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CLEC16A
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 23274
Swiss Prot Q2KHT3
Subcellular location Cytoplasm, Cell membrane
Synonyms Gop-1; KIAA0350; Protein CLEC16A; C-type lectin domain family 16 member A; CLEC16A
Background Regulator of mitophagy through the upstream regulation of the RNF41/NRDP1-PARK2 pathway. Mitophagy is a selective form of autophagy necessary for mitochondrial quality control. The RNF41/NRDP1-PARK2 pathway regulates autophagosome-lysosome fusion during late mitophagy. May protect RNF41/NRDP1 from proteosomal degradation, RNF41/NRDP1 which regulates proteosomal degradation of PARK2. Plays a key role in beta cells functions by regulating mitophagy/autophagy and mitochondrial health.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CLEC16A Polyclonal Antibody, PerCP Conjugated (bs-18539R-PerCP) validated for, and at what dilution?

bs-18539R-PerCP is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CLEC16A Polyclonal Antibody, PerCP Conjugated (bs-18539R-PerCP) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Pig.

How should bs-18539R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CLEC16A Polyclonal Antibody, PerCP Conjugated (bs-18539R-PerCP)?

KLH conjugated synthetic peptide derived from human CLEC16A

What are the excitation and emission wavelengths of the PerCP conjugate (bs-18539R-PerCP)?

PerCP on bs-18539R-PerCP: excitation / emission 482nm/677nm.