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Netrin 1 Polyclonal Antibody, APC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Cow
  • Pig
  • Chicken
Overview
Catalog # bs-1858R-APC
Product Name Netrin 1 Polyclonal Antibody, APC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Cow, Pig, Chicken
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NtN1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9423
Swiss Prot O95631
Subcellular location Secreted, Extracellular matrix
Synonyms NTN1L; Netrin-1; Epididymis tissue protein Li 131P; NTN1
Background Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Netrin 1 Polyclonal Antibody, APC Conjugated (bs-1858R-APC) validated for, and at what dilution?

bs-1858R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Netrin 1 Polyclonal Antibody, APC Conjugated (bs-1858R-APC) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Chicken.

How should bs-1858R-APC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Netrin 1 Polyclonal Antibody, APC Conjugated (bs-1858R-APC)?

KLH conjugated synthetic peptide derived from human NtN1

What are the excitation and emission wavelengths of the APC conjugate (bs-1858R-APC)?

APC on bs-1858R-APC: excitation / emission 650nm/660nm.