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phospho-MAK (Tyr159) Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-18634R-Cy7
Product Name phospho-MAK (Tyr159) Polyclonal Antibody, Cy7 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human MAK around the phosphorylation site of Tyr159
Modification Site Tyr159
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4117
Swiss Prot P20794
Subcellular location Cytoplasm, Nucleus
Synonyms RP62; dJ417M14.2; Serine/threonine-protein kinase MAK; Male germ cell-associated kinase; MAK
Background Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors (By similarity). Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR. Could play an important function in spermatogenesis. May play a role in chromosomal stability in prostate cancer cells.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is phospho-MAK (Tyr159) Polyclonal Antibody, Cy7 Conjugated (bs-18634R-Cy7) validated for, and at what dilution?

bs-18634R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does phospho-MAK (Tyr159) Polyclonal Antibody, Cy7 Conjugated (bs-18634R-Cy7) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-18634R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-MAK (Tyr159) Polyclonal Antibody, Cy7 Conjugated (bs-18634R-Cy7)?

KLH conjugated synthetic phosphopeptide derived from human MAK around the phosphorylation site of Tyr159

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-18634R-Cy7)?

Cy7 on bs-18634R-Cy7: excitation / emission 743nm/767nm.