phospho-MAK (Tyr159) Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-18634R-RBITC |
| Product Name | phospho-MAK (Tyr159) Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MAK around the phosphorylation site of Tyr159 |
| Modification Site | Tyr159 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4117 |
| Swiss Prot | P20794 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | RP62; dJ417M14.2; Serine/threonine-protein kinase MAK; Male germ cell-associated kinase; MAK |
| Background | Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors (By similarity). Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR. Could play an important function in spermatogenesis. May play a role in chromosomal stability in prostate cancer cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-MAK (Tyr159) Polyclonal Antibody, RBITC Conjugated (bs-18634R-RBITC) validated for, and at what dilution?
bs-18634R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-MAK (Tyr159) Polyclonal Antibody, RBITC Conjugated (bs-18634R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-18634R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-MAK (Tyr159) Polyclonal Antibody, RBITC Conjugated (bs-18634R-RBITC)?
KLH conjugated synthetic phosphopeptide derived from human MAK around the phosphorylation site of Tyr159
What are the excitation and emission wavelengths of the RBITC conjugate (bs-18634R-RBITC)?
RBITC on bs-18634R-RBITC: excitation / emission 540nm/625nm.