DNAJC3 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1863R-BF488 |
| Product Name | DNAJC3 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human p58ipk/DNAJC3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5611 |
| Swiss Prot | Q13217 |
| Subcellular location | Cytoplasm |
| Synonyms | DNAJC3; Protein Kinase Inhibitor P58; DnaJ homolog subfamily C member 3; HP58; Interferon induced, double stranded RNA activated protein kinase inhibitor; P58; p58 ipk ; P58IPK; PRKRI; Protein kinase inhibitor of 58 kDa; Protein kinase inhibitor p58. |
| Background | DNAJC3 may act as an inhibitor of both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2/PKR to catalyze phosphorylation of the EIF2A. It also functions as a Co-chaperone of HSPA8/HSC70 and is involved in the unfolded protein response (UPR) during ER stress. It has been reported that this protein stimulates the ATPase activity of HSPA8/HSC70.DNAJC3 is also involved in ativiral defense - it is activated in response to infection by influenza virus through the dissociation of DNAJB1. DNAJC3 is widely expressed and can be found at high levels in the testis and pancreas (expression in cell lines is variable). |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DNAJC3 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-1863R-BF488) validated for, and at what dilution?
bs-1863R-BF488 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DNAJC3 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-1863R-BF488) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Horse.
How should bs-1863R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DNAJC3 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-1863R-BF488)?
KLH conjugated synthetic peptide derived from human p58ipk/DNAJC3
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-1863R-BF488)?
AbBy Fluor-488 on bs-1863R-BF488: excitation / emission 499nm/519nm.