MCM-BP Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MCM-BP Polyclonal Antibody, Unconjugated (bs-18727R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MCM-BP Polyclonal Antibody, Unconjugated (bs-18727R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MCM-BP Polyclonal Antibody, Unconjugated (bs-18727R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MCM-BP Polyclonal Antibody, Unconjugated (bs-18727R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-18727R
Product Name	MCM-BP Polyclonal Antibody
Applications	IHC-P, IHC-F, IF
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MCM-BP
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	79892
Swiss Prot	Q9BTE3
Subcellular location	Nucleus
Synonyms	C10orf119 ; chromosome 10 open reading frame 119 ; MCM-binding protein ; MCM-BP ; mcmbp ; MCMBP_HUMAN ; Mini-chromosome maintenance complex-binding protein ; Minichromosome maintenance complex binding protein.
Background	Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion.
Application Dilution
IHC-P	1:200-400
IHC-F
IF