MyoD family inhibitor Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MyoD family inhibitor Polyclonal Antibody, Unconjugated (bs-18743R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MyoD family inhibitor Polyclonal Antibody, Unconjugated (bs-18743R) at 1:400 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MyoD family inhibitor Polyclonal Antibody, Unconjugated (bs-18743R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MyoD family inhibitor Polyclonal Antibody, Unconjugated (bs-18743R) at 1:400 overnight at 4\u00b0C, DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Cow
Sheep
Pig
Horse

Overview
Catalog #	bs-18743R
Product Name	MyoD family inhibitor Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Specificity	In Mice, there is possible cross-reactivity with Glycoprotein endo-alpha-1,2-mannosidase (67% homology with immunogen sequence; 53kDa)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Cow, Sheep, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MDFI
Immunogen Range	101-200/246
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4188
Swiss Prot	Q99750
Subcellular location	Cytoplasm, Nucleus
Synonyms	I MF; I mfa; Inhibitor of MyoD family a; Mdfi; MDFI_HUMAN; MyoD family inhibitor; Myogenic repressor I-mf; OTTHUMP00000016375; OTTHUMP00000016376; OTTHUMP00000196103; OTTHUMP00000196414; OTTHUMP00000196415.
Background	Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways (By similarity).
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500

Applications

Reactivity

Predicted Reactivity

Search our catalog of hundreds of thousands of products