Profilin 2 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1875R-BF488 |
| Product Name | Profilin 2 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody may detect Profilin 1 due to a 71% amino acid sequence similarity in the immunogen sequence. |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Pig, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat Profilin 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 81531 |
| Swiss Prot | Q9EPC6 |
| Subcellular location | Cytoplasm |
| Synonyms | Profilin-2; Profilin II; Pfn2 |
| Background | Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Profilin 2 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-1875R-BF488) validated for, and at what dilution?
bs-1875R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Profilin 2 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-1875R-BF488) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Pig, Chicken.
How should bs-1875R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Profilin 2 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-1875R-BF488)?
KLH conjugated synthetic peptide derived from rat Profilin 2
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-1875R-BF488)?
AbBy Fluor-488 on bs-1875R-BF488: excitation / emission 499nm/519nm.