Profilin 2 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1875R-RBITC |
| Product Name | Profilin 2 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody may detect Profilin 1 due to a 71% amino acid sequence similarity in the immunogen sequence. |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Pig, Chicken |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat Profilin 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 81531 |
| Swiss Prot | Q9EPC6 |
| Subcellular location | Cytoplasm |
| Synonyms | Profilin-2; Profilin II; Pfn2 |
| Background | Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Profilin 2 Polyclonal Antibody, RBITC Conjugated (bs-1875R-RBITC) validated for, and at what dilution?
bs-1875R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Profilin 2 Polyclonal Antibody, RBITC Conjugated (bs-1875R-RBITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Pig, Chicken.
How should bs-1875R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Profilin 2 Polyclonal Antibody, RBITC Conjugated (bs-1875R-RBITC)?
KLH conjugated synthetic peptide derived from rat Profilin 2
What are the excitation and emission wavelengths of the RBITC conjugate (bs-1875R-RBITC)?
RBITC on bs-1875R-RBITC: excitation / emission 540nm/625nm.