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MED18 Polyclonal Antibody, APC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-18764R-APC
Product Name MED18 Polyclonal Antibody, APC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MED18
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 54797
Swiss Prot Q9BUE0
Subcellular location Nucleus
Synonyms FLJ20045; hMED 18; hMED18; Human Mediator of RNA Polymerase II Transcription; MED 18; med18; MED18_HUMAN; Mediator complex subunit 18; Mediator of RNA polymerase II transcription subunit 18; Mediator of RNA polymerase II transcription subunit 18 homolog; OTTHUMP00000003645; p28b; TRAP/mediator complex subunit p28b.
Background MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM, Oct 2008]
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is MED18 Polyclonal Antibody, APC Conjugated (bs-18764R-APC) validated for, and at what dilution?

bs-18764R-APC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MED18 Polyclonal Antibody, APC Conjugated (bs-18764R-APC) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-18764R-APC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MED18 Polyclonal Antibody, APC Conjugated (bs-18764R-APC)?

KLH conjugated synthetic peptide derived from human MED18

What are the excitation and emission wavelengths of the APC conjugate (bs-18764R-APC)?

APC on bs-18764R-APC: excitation / emission 650nm/660nm.