MED18 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-18764R-BF750-100uL |
| Product Name | MED18 Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MED18 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 54797 |
| Swiss Prot | Q9BUE0 |
| Subcellular location | Nucleus |
| Synonyms | FLJ20045; hMED 18; hMED18; Human Mediator of RNA Polymerase II Transcription; MED 18; med18; MED18_HUMAN; Mediator complex subunit 18; Mediator of RNA polymerase II transcription subunit 18; Mediator of RNA polymerase II transcription subunit 18 homolog; OTTHUMP00000003645; p28b; TRAP/mediator complex subunit p28b. |
| Background | MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM, Oct 2008] |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MED18 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-18764R-BF750) validated for, and at what dilution?
bs-18764R-BF750 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MED18 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-18764R-BF750) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-18764R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MED18 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-18764R-BF750)?
KLH conjugated synthetic peptide derived from human MED18
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-18764R-BF750)?
AbBy Fluor-750 on bs-18764R-BF750: excitation / emission 749nm/775nm.