MED28 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-18767R-BF488 |
| Product Name | MED28 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MED28 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 610311 |
| Swiss Prot | Q9H204 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | EG1; Endothelial-derived protein 1; FKSG20; Magicin; med28; MED28_HUMAN; Mediator complex subunit 28; Mediator of RNA polymerase II transcription subunit 28; Mediator of RNA polymerase II transcription subunit 28; Mediator of RNA polymerase II transcription subunit 28; Merlin and Grb2-interacting cytoskeletal protein; Tumor angiogenesis marker EG-1. |
| Background | Med28 is a 178 amino acid subunit of the Mediator complex that localizes to both nucleus and cytoplasm. Widely expressed with highest expression in vascular tissues such as placenta, testis and liver, Med28 participates in the regulation of Pol II-mediated gene expression and is thought to play a key role in signal regulation and cell proliferation. It is suggested that Med28 functions as a repressor of SMC (smooth muscle cell) differentiation and may be associated with abnormalities in SMC growth and differentiation, including atherosclerosis, asthma, hypertension and smooth muscle tumors. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MED28 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-18767R-BF488) validated for, and at what dilution?
bs-18767R-BF488 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MED28 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-18767R-BF488) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish.
How should bs-18767R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MED28 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-18767R-BF488)?
KLH conjugated synthetic peptide derived from human MED28
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-18767R-BF488)?
AbBy Fluor-488 on bs-18767R-BF488: excitation / emission 499nm/519nm.