MGLUR5 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-18801R-Cy5.5 |
| Product Name | MGLUR5 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Sheep, Horse, Chicken, Monkey |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MGLUR5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2915 |
| Swiss Prot | P41594 |
| Subcellular location | Cell membrane |
| Synonyms | MGLUR5B; Glutamate receptor metabotropic 5; GPRC1E; GRM 5; GRM5; Metabotropic glutamate receptor 5; mGlu5; mGluR5; mGluR5a; mGluR5b; Metabotropic glutamate receptor 5 variant F; Metabotropic Glutamate Receptor 5b; Metabotropic glutamate receptor 5 variant G; Metabotropic glutamate receptor 5 variant H; PPP1R86; GRM5_HUMAN; |
| Background | Glutamate receptors constitute the principal excitatory neurotransmitter receptors in brain. Two classes of glutamate receptors exist: Ionotropic receptors, and metabotropic receptors (mGluRs). Metabotropic Glutamate Receptor 5b (GRM5) activity is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. The metabotropic glutamate receptor type 5 (mGlu5) is expressed in two splice variants, mGlu5a and mGlu5b, which differ in that mGlu5b has a 33-amino acid insert in the intracellular C-terminal domain. This receptor subtype is highly regulated, with higher levels found in developing animals. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is MGLUR5 Polyclonal Antibody, Cy5.5 Conjugated (BS-18801R-CY5-5) validated for, and at what dilution?
BS-18801R-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MGLUR5 Polyclonal Antibody, Cy5.5 Conjugated (BS-18801R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Horse, Chicken, Monkey.
How should BS-18801R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MGLUR5 Polyclonal Antibody, Cy5.5 Conjugated (BS-18801R-CY5-5)?
KLH conjugated synthetic peptide derived from human MGLUR5
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-18801R-CY5-5)?
Cy5.5 on BS-18801R-CY5-5: excitation / emission 675nm/694nm.