MetAP1 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MetAP1 Polyclonal Antibody, Unconjugated (bs-18806R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MetAP1 Polyclonal Antibody, Unconjugated (bs-18806R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MetAP1 Polyclonal Antibody, Unconjugated (bs-18806R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MetAP1 Polyclonal Antibody, Unconjugated (bs-18806R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Cow
Horse
Chicken
Rabbit

Overview
Catalog #	bs-18806R
Product Name	MetAP1 Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Cow, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MetAP1
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23173
Swiss Prot	P53582
Subcellular location	Cytoplasm
Synonyms	Methionyl Aminopeptidase 1; AMPM1_HUMAN; DKFZp781C0419; KIAA0094; MAP 1; MAP1A; MetAP-1; MetAP1; metap1; MetAP1A; Methionine aminopeptidase 1; Peptidase M 1.
Background	Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Required for normal progression through the cell cycle.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF