Securin/PTTG Polyclonal Antibody

$Lane 1: human colon carcinoma Lane 2: mouse brain lysates probed with Anti Securin\/PTTG Polyclonal Antibody, Unconjugated (bs-1881R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 22-28kD. Observed band size: 23kD$

Lane 1: human colon carcinoma Lane 2: mouse brain lysates probed with Anti Securin\/PTTG Polyclonal Antibody, Unconjugated (bs-1881R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 22-28kD. Observed band size: 23kD

Mouse testis lysates probed with Rabbit Anti-Securin Polyclonal Antibody, Unconjugated (bs-1881R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Securin\/PTTG Polyclonal Antibody, Unconjugated (bs-1881R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Securin\/PTTG Polyclonal Antibody, Unconjugated (bs-1881R) at 1:400 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Securin\/PTTG Polyclonal Antibody, Unconjugated (bs-1881R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Securin\/PTTG Polyclonal Antibody, Unconjugated (bs-1881R) at 1:400 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nSecurin\/PTTG Polyclonal Antibody, Unconjugated (bs-1881R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nSecurin\/PTTG Polyclonal Antibody, Unconjugated (bs-1881R) at 1:400 overnight at 4\u00b0C, DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Pig
Horse

Overview
Catalog #	bs-1881R
Product Name	Securin/PTTG Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PTTG
Immunogen Range	101-202/202
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9232
Subcellular location	Cytoplasm, Nucleus
Synonyms	AW555095; C87862; Cut2; EAP 1; EAP1; ESP1 ASSOCIATED PROTEIN 1; Esp1-associated protein; hPTTG; MGC126883; MGC138276; Pds1; Pituitary tumor transforming 1; Pituitary tumor transforming protein 1; Pituitary tumor-transforming 1, isoform CRA_a; Pituitary tumor-transforming 1, isoform CRA_b; Pituitary tumor-transforming gene 1; Pituitary tumor-transforming gene 1 protein; PTTG 1; PTTG; PTTG1; PTTG1 protein; Pttg3; TUMOR TRANSFORMING 1; Tumor transforming protein 1; TUMOR-TRANSFORMING 1; TUTR 1; TUTR1.
Background	Securin is an anaphase inhibitor and plays an important role in DNA damage and spindle check point pathways. It inhibits sister chromatid separation by binding and inhibiting Esp 1, a cystein protease that causes cleavage of the cohesin Scc 1 that binds the sister chromatids together. It is responsible for targeting of Esp 1 to the nucleus and its binding to the spindle. Degradation of Securin occurs shortly before anaphase, which liberates Esp 1 and is a pre-requisite for anaphase entry. It is targeted for degradation by ubiquitination mediated bycyclosome / anaphase promoting complex (APC)functioning as a ubiquitin ligase. In response to DNA damage Chk 1 phosphorylates Securin to stabilize it against the APC mediated destruction, hence preventing the entry of such a cell into anaphase. Securin is highly expressed in various human tumors, including pituitary, adrenal, kidney, liver, and ovarian tumors, and in cell lines derived from pituitary tumors, breast tumors, endometrial tumors, and ovarian tumors.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

Predicted Reactivity

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