MRas Polyclonal Antibody, APC Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-1882R-APC |
| Product Name | MRas Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Horse, Chicken |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MRAS |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 22808 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | muscle RAS oncogene homolog precursor; M ras; Muscle and microspikes Ras; Muscle Ras oncogene homologue; R ras3; R-ras3; Ras related protein MRas; Ras related protein RRas3; RRas3; XRas. |
| Background | MRas is a member of the RAS superfamily of GTP-binding proteins which are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors. MRas participates in reorganisation of actin cytoskeleton. It is also involved in focal adhesion and can activate MAPK signalling. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MRas Polyclonal Antibody, APC Conjugated (bs-1882R-APC) validated for, and at what dilution?
bs-1882R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-1882R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MRas Polyclonal Antibody, APC Conjugated (bs-1882R-APC)?
KLH conjugated synthetic peptide derived from human MRAS
What are the excitation and emission wavelengths of the APC conjugate (bs-1882R-APC)?
APC on bs-1882R-APC: excitation / emission 650nm/660nm.