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Reprimo Polyclonal Antibody, AbBy Fluor-647 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Cow
  • Rabbit
Overview
Catalog # bs-1885R-BF647
Product Name Reprimo Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Cow, Rabbit
Specifications
Conjugation AbBy Fluor-647
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Reprimo
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 56475
Swiss Prot Q9NS64
Subcellular location Cytoplasm
Synonyms REPRIMO; Protein reprimo; RPRM
Background May be involved in the regulation of p53-dependent G2 arrest of the cell cycle. Seems to induce cell cycle arrest by inhibiting CDK1 activity and nuclear translocation of the CDC2 cyclin B1 complex (By similarity).
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Reprimo Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-1885R-BF647) validated for, and at what dilution?

bs-1885R-BF647 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Reprimo Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-1885R-BF647) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Cow, Rabbit.

How should bs-1885R-BF647 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Reprimo Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-1885R-BF647)?

KLH conjugated synthetic peptide derived from human Reprimo

What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-1885R-BF647)?

AbBy Fluor-647 on bs-1885R-BF647: excitation / emission 650nm/665nm.