RSPO3 Polyclonal Antibody

$Lane 1: Human Hela cell lysates; Lane 2: Human Lovo cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Human Huvec cell lysates probed with RSPO3 Polyclonal Antibody, Unconjugated (bs-18877R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human Hela cell lysates; Lane 2: Human Lovo cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Human Huvec cell lysates probed with RSPO3 Polyclonal Antibody, Unconjugated (bs-18877R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Pig
Horse
Cat
Rabbit
GuineaPig

Overview
Catalog #	bs-18877R
Product Name	RSPO3 Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Pig, Horse, Cat, Rabbit, GuineaPig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RSPO3
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	84870
Swiss Prot	Q9BXY4
Subcellular location	Secreted
Synonyms	CRISTIN1; hPWTSR; hRspo3; Protein with TSP type-1 repeat; PWTSR; R-spondin 3 homolog; R-spondin-3 [Precursor]; R-spondin-3; Roof plate specific spondin-3; Roof plate-specific spondin-3; RSPO3_HUMAN; Thrombospondin type I domain containing 2; Thrombospondin type-1 domain-containing protein 2; THSD2.
Background	Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000