RSPO3 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-18877R-BF750-100uL |
| Product Name | RSPO3 Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse, Cat, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RSPO3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84870 |
| Swiss Prot | Q9BXY4 |
| Subcellular location | Secreted |
| Synonyms | CRISTIN1; hPWTSR; hRspo3; Protein with TSP type-1 repeat; PWTSR; R-spondin 3 homolog; R-spondin-3 [Precursor]; R-spondin-3; Roof plate specific spondin-3; Roof plate-specific spondin-3; RSPO3_HUMAN; Thrombospondin type I domain containing 2; Thrombospondin type-1 domain-containing protein 2; THSD2. |
| Background | Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is RSPO3 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-18877R-BF750) validated for, and at what dilution?
bs-18877R-BF750 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RSPO3 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-18877R-BF750) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Cat, Rabbit, GuineaPig.
How should bs-18877R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RSPO3 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-18877R-BF750)?
KLH conjugated synthetic peptide derived from human RSPO3
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-18877R-BF750)?
AbBy Fluor-750 on bs-18877R-BF750: excitation / emission 749nm/775nm.