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TMEM175 Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
Overview
Catalog # bs-18922R-Biotin
Product Name TMEM175 Polyclonal Antibody, Biotin Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Mouse, Rat
Predicted Reactivity Human
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TM175
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 84286
Swiss Prot Q9BSA9
Subcellular location Cell membrane
Synonyms MGC4618; Hypothetical protein DKFZp547K246; hypothetical protein LOC84286; Hypothetical protein MGC4618; TM175_HUMAN; tmem175; Transmembrane protein 175.
Background Organelle-specific potassium channel specifically responsible for potassium conductance in endosomes and lysosomes. Forms a potassium-permeable leak-like channel, which regulates lumenal pH stability and is required for autophagosome-lysosome fusion. Constitutes the major lysosomal potassium channel.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is TMEM175 Polyclonal Antibody, Biotin Conjugated (bs-18922R-Biotin) validated for, and at what dilution?

bs-18922R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TMEM175 Polyclonal Antibody, Biotin Conjugated (bs-18922R-Biotin) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.

How should bs-18922R-Biotin be stored?

Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TMEM175 Polyclonal Antibody, Biotin Conjugated (bs-18922R-Biotin)?

KLH conjugated synthetic peptide derived from human TM175