TMEM175 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-18922R-PerCP-Cy5.5 |
| Product Name | TMEM175 Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TM175 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84286 |
| Swiss Prot | Q9BSA9 |
| Subcellular location | Cell membrane |
| Synonyms | MGC4618; Hypothetical protein DKFZp547K246; hypothetical protein LOC84286; Hypothetical protein MGC4618; TM175_HUMAN; tmem175; Transmembrane protein 175. |
| Background | Organelle-specific potassium channel specifically responsible for potassium conductance in endosomes and lysosomes. Forms a potassium-permeable leak-like channel, which regulates lumenal pH stability and is required for autophagosome-lysosome fusion. Constitutes the major lysosomal potassium channel. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TMEM175 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-18922R-PERCP-CY5-5) validated for, and at what dilution?
BS-18922R-PERCP-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TMEM175 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-18922R-PERCP-CY5-5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should BS-18922R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TMEM175 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-18922R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human TM175
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-18922R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-18922R-PERCP-CY5-5: excitation / emission 482nm/694nm.