GRM4 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1894R-Cy3 |
| Product Name | GRM4 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody detects Metabotropic Glutamate Receptor 4. It may also detect Metabotropic Glutamate Receptor 6 due to sequence similarity. |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Metabotropic Glutamate Receptor 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2914 |
| Swiss Prot | Q14833 |
| Subcellular location | Cell membrane |
| Synonyms | mGlu4; GPRC1D; MGLUR4; Metabotropic glutamate receptor 4; GRM4 |
| Background | G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GRM4 Polyclonal Antibody, Cy3 Conjugated (bs-1894R-Cy3) validated for, and at what dilution?
bs-1894R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GRM4 Polyclonal Antibody, Cy3 Conjugated (bs-1894R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Pig, Rabbit.
How should bs-1894R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GRM4 Polyclonal Antibody, Cy3 Conjugated (bs-1894R-Cy3)?
KLH conjugated synthetic peptide derived from human Metabotropic Glutamate Receptor 4
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-1894R-Cy3)?
Cy3 on bs-1894R-Cy3: excitation / emission 512,550nm/570,615nm.