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N ras + c Ha ras Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with N ras + c Ha ras Polyclonal Antibody, Unconjugated (bs-18980R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with N ras + c Ha ras Polyclonal Antibody, Unconjugated (bs-18980R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with N ras + c Ha ras Polyclonal Antibody, Unconjugated (bs-18980R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with N ras + c Ha ras Polyclonal Antibody, Unconjugated (bs-18980R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (N ras + c Ha ras) Polyclonal Antibody, Unconjugated (bs-18980R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (N ras + c Ha ras) Polyclonal Antibody, Unconjugated (bs-18980R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Bioss unconjugated antibody vial

Applications

  • IHC-P
  • IHC-F
  • IF(ICC)
  • IF

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Pig
Overview
Catalog # bs-18980R
Product Name N ras + c Ha ras Polyclonal Antibody
Applications
IHC-P IHC-F IF(ICC) IF
Reactivity Human, Mouse, Rat
Predicted Reactivity Pig
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human N ras + c Ha ras
Immunogen Range 101-189/189
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 3265
Swiss Prot P01111
Synonyms ALPS4; GTPase HRas; GTPase NRas; HRAS1; N ras protein part 4; Neuroblastoma RAS viral (v-ras) oncogene homolog; NRAS; Ras family small GTP binding protein H Ras; RAS1; Transforming protein p21.
Background This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq, Jul 2008]
Application Dilution
IHC-P 1:200-400
IHC-F 1:100-500
IF(ICC) 1:50-200
IF

Frequently asked questions

Which applications is N ras + c Ha ras Polyclonal Antibody (bs-18980R) validated for, and at what dilution?

bs-18980R is validated for IHC-P (1:200-400); IHC-F (1:100-500); IF(ICC) (1:50-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does N ras + c Ha ras Polyclonal Antibody (bs-18980R) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Pig.

How should bs-18980R be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise N ras + c Ha ras Polyclonal Antibody (bs-18980R)?

KLH conjugated synthetic peptide derived from human N ras + c Ha ras Immunogen range: 101-189/189.

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