N ras + c Ha ras Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-18980R-BF488 |
| Product Name | N ras + c Ha ras Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
IF(ICC)
IF
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Pig |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human N ras + c Ha ras |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3265 |
| Swiss Prot | P01111 |
| Synonyms | ALPS4; GTPase HRas; GTPase NRas; HRAS1; N ras protein part 4; Neuroblastoma RAS viral (v-ras) oncogene homolog; NRAS; Ras family small GTP binding protein H Ras; RAS1; Transforming protein p21. |
| Background | This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| IF(ICC) | 1:50-200 |
| IF | |
Frequently asked questions
Which applications is N ras + c Ha ras Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-18980R-BF488) validated for, and at what dilution?
bs-18980R-BF488 is validated for IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does N ras + c Ha ras Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-18980R-BF488) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Pig.
How should bs-18980R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise N ras + c Ha ras Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-18980R-BF488)?
KLH conjugated synthetic peptide derived from human N ras + c Ha ras
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-18980R-BF488)?
AbBy Fluor-488 on bs-18980R-BF488: excitation / emission 499nm/519nm.