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NARS Polyclonal Antibody, AbBy Fluor-594 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-19023R-BF594-100uL
Product Name NARS Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation AbBy Fluor-594
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NARS
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4677
Swiss Prot O43776
Subcellular location Cytoplasm
Synonyms 3010001M15Rik; AA960128; AsnRS; ASNS; Asparagine tRNA ligase 1, cytoplasmic; Asparagine tRNA ligase; Asparagine--tRNA ligase; Asparaginyl tRNA synthetase; Asparaginyl-tRNA synthetase; C78150; cytoplasmic; EC 6.1.1.22; LRRGT00113; MGC116236; Nars; NARS1; NRS; SYNC_HUMAN.
Background Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases. [provided by RefSeq, Jul 2008]
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is NARS Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-19023R-BF594) validated for, and at what dilution?

bs-19023R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NARS Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-19023R-BF594) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse.

How should bs-19023R-BF594 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NARS Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-19023R-BF594)?

KLH conjugated synthetic peptide derived from human NARS

What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-19023R-BF594)?

AbBy Fluor-594 on bs-19023R-BF594: excitation / emission 590nm/617nm.