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NARS Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-19023R-FITC
Product Name NARS Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NARS
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4677
Swiss Prot O43776
Subcellular location Cytoplasm
Synonyms 3010001M15Rik; AA960128; AsnRS; ASNS; Asparagine tRNA ligase 1, cytoplasmic; Asparagine tRNA ligase; Asparagine--tRNA ligase; Asparaginyl tRNA synthetase; Asparaginyl-tRNA synthetase; C78150; cytoplasmic; EC 6.1.1.22; LRRGT00113; MGC116236; Nars; NARS1; NRS; SYNC_HUMAN.
Background Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases. [provided by RefSeq, Jul 2008]
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is NARS Polyclonal Antibody, FITC Conjugated (bs-19023R-FITC) validated for, and at what dilution?

bs-19023R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NARS Polyclonal Antibody, FITC Conjugated (bs-19023R-FITC) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse.

How should bs-19023R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NARS Polyclonal Antibody, FITC Conjugated (bs-19023R-FITC)?

KLH conjugated synthetic peptide derived from human NARS

What are the excitation and emission wavelengths of the FITC conjugate (bs-19023R-FITC)?

FITC on bs-19023R-FITC: excitation / emission 494nm/518nm.