NASP Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NASP Polyclonal Antibody, Unconjugated (bs-19026R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NASP Polyclonal Antibody, Unconjugated (bs-19026R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Cow
Sheep
Horse
Rabbit

Overview
Catalog #	bs-19026r
Product Name	NASP Polyclonal Antibody
Applications	IHC-P IHC-F IF
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Cow, Sheep, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NASP
Immunogen Range	2-100/788
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4678
Swiss Prot	P49321
Subcellular location	Cytoplasm, Nucleus
Synonyms	5033430J04Rik; AI131596; AI317140; D4Ertd767e; DKFZp547F162; Epcs32; FLB7527; FLJ31599; FLJ35510; MGC19722; MGC20372; MGC2297; MGC93869; NASP; NASP_HUMAN; nuclear autoantigenic sperm protein (histone binding); Nuclear autoantigenic sperm protein; PRO1999; RP23 233B9.9.
Background	Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.
Application Dilution
IHC-P	=1:100-500
IHC-F	IHC-F=1:100-500
IF	IF=1:100-500