NCOA4 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NCOA4 Polyclonal Antibody, Unconjugated (bs-19051R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NCOA4 Polyclonal Antibody, Unconjugated (bs-19051R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Lane 1: Human HT1080 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human U937 cell lysates probed with NCOA4 Polyclonal Antibody, Unconjugated (bs-19051R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Human HT1080 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human U937 cell lysates probed with NCOA4 Polyclonal Antibody, Unconjugated (bs-19051R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

ELISA
IHC-P
IHC-F
IF

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Cow
Sheep
Pig
Horse

Overview
Catalog #	bs-19051R
Product Name	NCOA4 Polyclonal Antibody
Applications	ELISA IHC-P IHC-F IF
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Cow, Sheep, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NCOA4
Immunogen Range	1-100/614
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8031
Swiss Prot	Q13772
Subcellular location	Nucleus
Synonyms	70 kDa androgen receptor activator; 70 kDa androgen receptor coactivator; 70 kDa AR activator; 70 kDa AR-activator; Androgen receptor coactivator 70 kD; Androgen receptor coactivator 70 kDa protein; Androgen receptor-associated protein of 70 kDa; ARA 70; ARA70; DKFZp762E1112; ELE 1; ELE1; ELE1/ret TK; NCOA 4; NCoA-4; NCOA4_HUMAN; Nuclear receptor coactivator 4; Papillary thyroid carcinoma 3; PTC 3; PTC3; RET activating gene ELE1; Ret activating protein ELE1; Ret-activating protein ELE1; RFG.
Background	Enhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF