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NDFIP2 Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • IF(ICC)
  • IF

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-19059R-PE
Product Name NDFIP2 Polyclonal Antibody, PE Conjugated
Applications
WB IF(ICC) IF
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NDFIP2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 54602
Swiss Prot Q9NV92
Synonyms Ndfip2; NEDD4 family interacting protein 2; NEDD4 family-interacting protein 2; NEDD4 WW domain binding protein 5A; NEDD4 WW domain-binding protein 5A; NFIP2_HUMAN; Putative MAPK activating protein PM04/PM05/PM06/PM07; Putative MAPK-activating protein PM04/PM05/PM06/PM07; Putative NF kappa B activating protein 413; Putative NF-kappa-B-activating protein 413.
Background Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. May modulate EGFR signaling.
Application Dilution
WB 1:300-5000
IF(ICC)
IF

Frequently asked questions

Which applications is NDFIP2 Polyclonal Antibody, PE Conjugated (bs-19059R-PE) validated for, and at what dilution?

bs-19059R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NDFIP2 Polyclonal Antibody, PE Conjugated (bs-19059R-PE) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.

How should bs-19059R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NDFIP2 Polyclonal Antibody, PE Conjugated (bs-19059R-PE)?

KLH conjugated synthetic peptide derived from human NDFIP2

What are the excitation and emission wavelengths of the PE conjugate (bs-19059R-PE)?

PE on bs-19059R-PE: excitation / emission 496,564nm/578nm.