Help

TIPRL Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
  • GuineaPig
Overview
Catalog # bs-19121R-FITC
Product Name TIPRL Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Pig, Horse, Rabbit, GuineaPig
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TIPRL
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 26172
Swiss Prot O75663
Subcellular location Cytoplasm
Synonyms TIP41-like protein; TIPRL; Putative MAPK-activating protein PM10; Type 2A-interacting protein; TIP
Background May be a allosteric regulator of serine/threonine-protein phosphatase 2A (PP2A). Isoform 1 inhibits catalytic activity of the PP2A(D) core complex in vitro. The PP2A(C):TIPRL complex does not show phosphatase activity. May play a role in the regulation of ATM/ATR signaling pathway controlling DNA replication and repair.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is TIPRL Polyclonal Antibody, FITC Conjugated (bs-19121R-FITC) validated for, and at what dilution?

bs-19121R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TIPRL Polyclonal Antibody, FITC Conjugated (bs-19121R-FITC) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Rabbit, GuineaPig.

How should bs-19121R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TIPRL Polyclonal Antibody, FITC Conjugated (bs-19121R-FITC)?

KLH conjugated synthetic peptide derived from human TIPRL

What are the excitation and emission wavelengths of the FITC conjugate (bs-19121R-FITC)?

FITC on bs-19121R-FITC: excitation / emission 494nm/518nm.