ZFPL1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-19136R-Cy3-100uL |
| Product Name | ZFPL1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZFPL1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7542 |
| Swiss Prot | O95159 |
| Subcellular location | Nucleus |
| Synonyms | D11S750; MCG4; MGC 4; ZFPL 1; Zinc finger protein like 1; Zinc finger protein MCG4; zinc-finger protein in MEN1 region. |
| Background | Zinc finger protein-like 1 is a 310 amino acid single-pass membrane protein with two zinc fingers at the N-terminus, the second of which is likely a RING domain. The RING domain, which is a 40-60 amino acid, cysteine-rich domain that binds two atoms of zinc, plays a key role in the ubiquitination pathway. The presence of zinc finger-like and leucine zipper-like domains in ZFPL1 suggests a role in DNA binding and transcriptional regulation. ZFP1 is widely expressed in the golgi apparatus and is involved in maintaining golgi structure and regulating the rate of cargo transport. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ZFPL1 Polyclonal Antibody, Cy3 Conjugated (bs-19136R-Cy3) validated for, and at what dilution?
bs-19136R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ZFPL1 Polyclonal Antibody, Cy3 Conjugated (bs-19136R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Pig, Horse, Rabbit.
How should bs-19136R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZFPL1 Polyclonal Antibody, Cy3 Conjugated (bs-19136R-Cy3)?
KLH conjugated synthetic peptide derived from human ZFPL1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-19136R-Cy3)?
Cy3 on bs-19136R-Cy3: excitation / emission 512,550nm/570,615nm.