phospho-Nephrin (Tyr1217) Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-19199R-BF647-100uL |
| Product Name | phospho-Nephrin (Tyr1217) Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
|
| Specificity | This phosphorylation site is homologous to that of Tyr1232 in Mouse and Tyr1228 Rat. |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Nephrin around the phosphorylation site of Tyr1217. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4868 |
| Swiss Prot | O60500 |
| Subcellular location | Cell membrane |
| Synonyms | CNF; NPHN; nephrin; Renal glomerulus-specific cell adhesion receptor; NPHS1 |
| Background | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is phospho-Nephrin (Tyr1217) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-19199R-BF647) validated for, and at what dilution?
bs-19199R-BF647 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Nephrin (Tyr1217) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-19199R-BF647) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bs-19199R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Nephrin (Tyr1217) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-19199R-BF647)?
KLH conjugated synthetic phosphopeptide derived from human Nephrin around the phosphorylation site of Tyr1217.
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-19199R-BF647)?
AbBy Fluor-647 on bs-19199R-BF647: excitation / emission 650nm/665nm.