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TLR1 Polyclonal Antibody, AbBy Fluor-350 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Horse
Overview
Catalog # bs-1919R-BF350-100uL
Product Name TLR1 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Dog, Cow, Horse
Specifications
Conjugation AbBy Fluor-350
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TLR1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7096
Swiss Prot Q15399
Subcellular location Cytoplasm, Cell membrane
Synonyms TIL; CD281; rsc786; TIL. LPRS5; Toll-like receptor 1; Toll/interleukin-1 receptor-like protein; TLR1; KIAA12
Background Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity).
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is TLR1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-1919R-BF350) validated for, and at what dilution?

bs-1919R-BF350 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TLR1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-1919R-BF350) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Horse.

How should bs-1919R-BF350 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TLR1 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-1919R-BF350)?

KLH conjugated synthetic peptide derived from human TLR1

What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-1919R-BF350)?

AbBy Fluor-350 on bs-1919R-BF350: excitation / emission 346nm/442nm.