Nesprin 1 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-19207R-BF750-100uL |
| Product Name | Nesprin 1 Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Nesprin 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23345 |
| Swiss Prot | Q8NF91 |
| Subcellular location | Cytoplasm |
| Synonyms | CPG2; Enaptin; Myne-1; MYNE1; Myocyte nuclear envelope protein 1; Nesprin-1; Nuclear envelope spectrin repeat protein 1; SCAR8; Synaptic nuclear envelope protein 1; Syne-1; SYNE1; SYNE1_HUMAN; SYNE1B. |
| Background | Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Connects nuclei to the cytoskeleton by interacting with the nuclear envelope and with F-actin in the cytoplasm. Required for centrosome migration to the apical cell surface during early ciliogenesis. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Nesprin 1 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-19207R-BF750) validated for, and at what dilution?
bs-19207R-BF750 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Nesprin 1 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-19207R-BF750) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Horse.
How should bs-19207R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Nesprin 1 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-19207R-BF750)?
KLH conjugated synthetic peptide derived from human Nesprin 1
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-19207R-BF750)?
AbBy Fluor-750 on bs-19207R-BF750: excitation / emission 749nm/775nm.