Nesprin 3 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-19209R-BF750-100uL |
| Product Name | Nesprin 3 Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Nesprin 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 161176 |
| Swiss Prot | Q6ZMZ3 |
| Subcellular location | Nucleus |
| Synonyms | C14orf49; Chromosome 14 open reading frame 49; FLJ25605; MGC75267; Nesp3; Nesprin3; Nesprin-3; NET53; Nuclear Envelope Spectrin Repeat Protein 3; Spectrin Repeat Containing, Nuclear Envelope Family Member 3; SYNE3; SYNE3_HUMAN. |
| Background | As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Nesprin 3 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-19209R-BF750) validated for, and at what dilution?
bs-19209R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Nesprin 3 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-19209R-BF750) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-19209R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Nesprin 3 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-19209R-BF750)?
KLH conjugated synthetic peptide derived from human Nesprin 3
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-19209R-BF750)?
AbBy Fluor-750 on bs-19209R-BF750: excitation / emission 749nm/775nm.