Help

NHP2 Polyclonal Antibody, AbBy Fluor-350 Conjugated

Applications

  • IF(ICC)
  • IF

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Horse
  • Rabbit
Overview
Catalog # bs-19235R-BF350-100uL
Product Name NHP2 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
IF(ICC) IF
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Horse, Rabbit
Specifications
Conjugation AbBy Fluor-350
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NHP2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 55651
Swiss Prot Q9NX24
Synonyms DKCB2; FLJ20479; H/ACA ribonucleoprotein complex subunit 2; NHP2; NHP2 like protein; NHP2 ribonucleoprotein; NHP2 ribonucleoprotein homolog (yeast) 1; NHP2 ribonucleoprotein homolog; NHP2, S. cerevisiae, homolog of; NHP2_HUMAN; NHP2P; NOLA2; Nucleolar protein family A member 2 (H/ACA small nucleolar RNPs); Nucleolar protein family A member 2; snoRNP protein NHP2.
Background This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA1 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The four H/ACA snoRNP proteins are also components of the telomerase complex. This gene encodes a protein related to Saccharomyces cerevisiae Nhp2p. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]
Application Dilution
IF(ICC) 1:50-200
IF

Frequently asked questions

Which applications is NHP2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-19235R-BF350) validated for, and at what dilution?

bs-19235R-BF350 is validated for IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NHP2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-19235R-BF350) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Horse, Rabbit.

How should bs-19235R-BF350 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NHP2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-19235R-BF350)?

KLH conjugated synthetic peptide derived from human NHP2

What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-19235R-BF350)?

AbBy Fluor-350 on bs-19235R-BF350: excitation / emission 346nm/442nm.