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NIPSNAP1 Polyclonal Antibody, AbBy Fluor-647 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-19252R-BF647
Product Name NIPSNAP1 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation AbBy Fluor-647
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NIPSNAP1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8508
Swiss Prot Q9BPW8
Subcellular location Cell membrane
Synonyms NIPSNAP 1; Nipsnap homolog 1 (C. elegans); Protein NipSnap homolog 1.
Background This gene encodes a member of the NipSnap family of proteins that may be involved in vesicular transport. A similar protein in mice inhibits the calcium channel TRPV6, and is also localized to the inner mitochondrial membrane where it may play a role in mitochondrial DNA maintenance. A pseudogene of this gene is located on the short arm of chromosome 17. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2011]
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is NIPSNAP1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-19252R-BF647) validated for, and at what dilution?

bs-19252R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NIPSNAP1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-19252R-BF647) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-19252R-BF647 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NIPSNAP1 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-19252R-BF647)?

KLH conjugated synthetic peptide derived from human NIPSNAP1

What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-19252R-BF647)?

AbBy Fluor-647 on bs-19252R-BF647: excitation / emission 650nm/665nm.