phospho-nNOS (Ser1417) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-19301R-Cy3-100uL |
| Product Name | phospho-nNOS (Ser1417) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat, Xenopus laevis |
| Predicted Reactivity | Mouse, Zebrafish |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated Synthesised phosphopeptide derived from human nNOS around the phosphorylation site of Ser1417 |
| Modification Site | Ser1417 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 842 |
| Swiss Prot | P55211 |
| Subcellular location | Cell membrane |
| Synonyms | MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; ICE-like apoptotic protease 6; CASP9 |
| Background | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-nNOS (Ser1417) Polyclonal Antibody, Cy3 Conjugated (bs-19301R-Cy3) validated for, and at what dilution?
bs-19301R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-nNOS (Ser1417) Polyclonal Antibody, Cy3 Conjugated (bs-19301R-Cy3) react with?
Tested reactivity: Human, Rat, Xenopus laevis. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Zebrafish.
How should bs-19301R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-nNOS (Ser1417) Polyclonal Antibody, Cy3 Conjugated (bs-19301R-Cy3)?
KLH conjugated Synthesised phosphopeptide derived from human nNOS around the phosphorylation site of Ser1417
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-19301R-Cy3)?
Cy3 on bs-19301R-Cy3: excitation / emission 512,550nm/570,615nm.