ZW10 Polyclonal Antibody, HRP Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-1938R-HRP-100uL |
| Product Name | ZW10 Polyclonal Antibody, HRP Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse ZW10 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9183 |
| Subcellular location | Cytoplasm |
| Synonyms | Centromere/kinetochore protein zw10; Centromere/kinetochore protein zw10 homolog; HZW 10; HZW10; Kinetochore associated homolog; KNTC1AP; MGC149821; Zeste White 10; Zeste white 10 homolog; ZW 10; ZW 10; centromere/kinetochore protein; ZW 10 kinetochore associated homolog; ZW10 Drosophila homolog centromere/kinetochore protein; ZW10 homolog centromere/kinetochore protein Drosophila; ZW10 homolog centromere/kinetochore protein; ZW10 kinetochore associated homolog; ZW10_MOUSE. |
| Background | The mitotic checkpoint ensures that chromosomes are divided equally between daughter cells and is a primary mechanism preventing the chromosome instability often seen in aneuploid human tumors. This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. The encoded protein binds to centromeres during the prophase, metaphase, and early anaphase cell division stages and to kinetochore microtubules during metaphase. It is part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. In mitotic human cells ZW10 resides in a complex with Rod and Zwilch, whereas another ZW10 partner, Zwint-1, is part of a separate complex of structural kinetochore components including Mis12 and Ndc80-Hec1. Zwint-1 is critical for recruiting ZW10 to unattached kinetochores. Depletion from human cells demonstrates that the ZW10 complex is essential for stable binding of a Mad1-Mad2 complex to unattached kinetochores. Thus, ZW10 functions as a linker between the core structural elements of the outer kinetochore and components that catalyze generation of the mitotic checkpoint-derived "stop anaphase" inhibitor. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is ZW10 Polyclonal Antibody, HRP Conjugated (bs-1938R-HRP) validated for, and at what dilution?
bs-1938R-HRP is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-1938R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZW10 Polyclonal Antibody, HRP Conjugated (bs-1938R-HRP)?
KLH conjugated synthetic peptide derived from mouse ZW10