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SAPS3 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Pig
  • Rabbit
  • Hamster
Overview
Catalog # bs-19390R-Cy3
Product Name SAPS3 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Pig, Rabbit, Hamster
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SAPS3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 55291
Swiss Prot Q5H9R7
Subcellular location Cytoplasm, Nucleus
Synonyms C11orf23; Chromosome 11 open reading frame 23; KIAA1558; PP6R3; PP6R3_HUMAN; PPP6R3; Protein phosphatase 6 regulatory subunit 3; SAP190; SAPL; SAPLa; SAPS domain family member 3; SAPS domain family, member 3; Serine/threonine-protein phosphatase 6 regulatory subunit 3; Sporulation induced transcript 4 associated protein SAPL; Sporulation-induced transcript 4-associated protein SAPL.
Background Protein phosphatase regulatory subunits, such as SAPS3, modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity, recruiting substrates, and determining the intracellular localization of the holoenzyme. SAPS3 is a regulatory subunit for the protein phosphatase-6 catalytic subunit (PPP6C; MIM 612725) (Stefansson and Brautigan, 2006 [PubMed 16769727]).[supplied by OMIM, Nov 2010]
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is SAPS3 Polyclonal Antibody, Cy3 Conjugated (bs-19390R-Cy3) validated for, and at what dilution?

bs-19390R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SAPS3 Polyclonal Antibody, Cy3 Conjugated (bs-19390R-Cy3) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Pig, Rabbit, Hamster.

How should bs-19390R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SAPS3 Polyclonal Antibody, Cy3 Conjugated (bs-19390R-Cy3)?

KLH conjugated synthetic peptide derived from human SAPS3

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-19390R-Cy3)?

Cy3 on bs-19390R-Cy3: excitation / emission 512,550nm/570,615nm.