SARA Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-19393R-BF488 |
| Product Name | SARA Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SARA |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9372 |
| Swiss Prot | O95405 |
| Subcellular location | Cytoplasm |
| Synonyms | NSP; SARA; MADHIP; SMADIP; PPP1R173; Zinc finger FYVE domain-containing protein 9; Mothers against decapentaplegic homolog-interacting protein; Madh-interacting protein; Novel serine protease; Receptor activation anchor; hSARA; Smad anchor for receptor activation; ZFYVE9 |
| Background | Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SARA Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-19393R-BF488) validated for, and at what dilution?
bs-19393R-BF488 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SARA Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-19393R-BF488) react with?
Tested reactivity: Human.
How should bs-19393R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SARA Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-19393R-BF488)?
KLH conjugated synthetic peptide derived from human SARA
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-19393R-BF488)?
AbBy Fluor-488 on bs-19393R-BF488: excitation / emission 499nm/519nm.