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ZNF307/ZKSCAN3 Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse kidney labeled with Anti ZNF307 Polyclonal Antibody (bs-1940R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse kidney labeled with Anti ZNF307 Polyclonal Antibody (bs-1940R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ZNF307\/ZKSCAN3) polyclonal Antibody, Unconjugated (bs-1940R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ZNF307\/ZKSCAN3) polyclonal Antibody, Unconjugated (bs-1940R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
SH-SY5Y cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with ZNF307\/ZKSCAN3 Antibody(bs-1940R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
SH-SY5Y cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with ZNF307\/ZKSCAN3 Antibody(bs-1940R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-1940R-100uL
Product Name ZNF307/ZKSCAN3 Polyclonal Antibody
Applications
WB IHC-P IHC-F IF FLOW-CYT ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ZNF307
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 387032
Synonyms zinc finger with KRAB and SCAN domains 4; Zinc finger protein 307; P373c6.1; ZKSCAN4; FLJ32136; P1P373C6; p373c6.1 novel C2H2 type zinc finger protein; p373c6.1; Zinc finger protein 307; Zinc finger with KRAB and SCAN domains 4; ZKSCAN4; ZKSC4_HUMAN.
Background ZNF307 contains 1 SCAN box domain, 1 KRAB domain and 7 C2H2-type zinc fingers. It belongs to the Krueppel C2H2-type zinc-finger protein family and may be involved in transcriptional regulation. [SUBCELLULAR LOCATION] Nucleus. Expressed in adult heart, brain, placenta, lung and kidney, but not in adult liver and skeletal muscle. In 17-day old embryo, detected in liver, skeletal muscle, brain, heart and small intestine.
Application Dilution
WB FCM1:100
IHC-P IFICC1:100
IHC-F
IF
Flow-Cyt
ICC/IF

Frequently asked questions

Which applications is ZNF307/ZKSCAN3 Polyclonal Antibody (bs-1940R) validated for, and at what dilution?

bs-1940R is validated for FCM (1:100); IF(ICC) (1:100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ZNF307/ZKSCAN3 Polyclonal Antibody (bs-1940R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-1940R be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ZNF307/ZKSCAN3 Polyclonal Antibody (bs-1940R)?

KLH conjugated synthetic peptide derived from human ZNF307

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