WRCH1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1944R-Cy3 |
| Product Name | WRCH1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human WRCH-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 58480 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Wnt-1 responsive Cdc42 homolog 1; Rho-related GTP-binding protein RhoU; Ryu GTPase; CDC42-like GTPase 1; Rho GTPase-like protein ARHU; GTP-binding protein-like 1; RHOU; SB128; ARHU; CDC42L1; G28K; WRCH1; RHOU_HUMAN. |
| Background | The WRCH1 protein is a member of the Rho family of GTPases. This protein can activate PAK1 and JNK1, and can induce filopodium formation and stress fiber dissolution. It may also mediate the effects of WNT1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is WRCH1 Polyclonal Antibody, Cy3 Conjugated (bs-1944R-Cy3) validated for, and at what dilution?
bs-1944R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does WRCH1 Polyclonal Antibody, Cy3 Conjugated (bs-1944R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Rabbit.
How should bs-1944R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise WRCH1 Polyclonal Antibody, Cy3 Conjugated (bs-1944R-Cy3)?
KLH conjugated synthetic peptide derived from human WRCH-1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-1944R-Cy3)?
Cy3 on bs-1944R-Cy3: excitation / emission 512,550nm/570,615nm.