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OIT3 Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Cow
Overview
Catalog # bs-19567R-FITC
Product Name OIT3 Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Cow
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human OIT3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 170392
Swiss Prot Q8WWZ8
Subcellular location Nucleus
Synonyms Liver specific zona pellucida domain containing protein; Liver specific ZP domain containing protein; LZP; OIT3_HUMAN; Oncoprotein induced transcript 3.
Background OIT3 contains one EGF-like domain and one ZP domain. It may be involved in hepatocellular function and development. OIT3 is liver-specific and is expressed only in the hepatocytes; down-regulated in hepatocellular carcinoma (HCC) and HCC cell lines. There are two named isoforms.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is OIT3 Polyclonal Antibody, FITC Conjugated (bs-19567R-FITC) validated for, and at what dilution?

bs-19567R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does OIT3 Polyclonal Antibody, FITC Conjugated (bs-19567R-FITC) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow.

How should bs-19567R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise OIT3 Polyclonal Antibody, FITC Conjugated (bs-19567R-FITC)?

KLH conjugated synthetic peptide derived from human OIT3

What are the excitation and emission wavelengths of the FITC conjugate (bs-19567R-FITC)?

FITC on bs-19567R-FITC: excitation / emission 494nm/518nm.