NUPL1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-19569R-Cy3 |
| Product Name | NUPL1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NUPL1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9818 |
| Swiss Prot | Q9BVL2 |
| Subcellular location | Nucleus |
| Synonyms | KIAA0410; Nucleoporin like 1; Nucleoporin p58/p45; NUPL 1; OTTHUMP00000018146; OTTHUMP00000042323; PRO2463. |
| Background | This gene encodes a member of the nucleoporin family that shares 87% sequence identity with rat nucleoporin p58. The protein is localized to the nuclear rim and is a component of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NUPL1 Polyclonal Antibody, Cy3 Conjugated (bs-19569R-Cy3) validated for, and at what dilution?
bs-19569R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NUPL1 Polyclonal Antibody, Cy3 Conjugated (bs-19569R-Cy3) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Horse, Chicken, Rabbit.
How should bs-19569R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NUPL1 Polyclonal Antibody, Cy3 Conjugated (bs-19569R-Cy3)?
KLH conjugated synthetic peptide derived from human NUPL1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-19569R-Cy3)?
Cy3 on bs-19569R-Cy3: excitation / emission 512,550nm/570,615nm.