Help

PSME1 Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat
  • Cynomolgus Monkey

Predicted Reactivity

  • Human
  • Cow
  • Pig
Overview
Catalog # bs-19575R-Cy7
Product Name PSME1 Polyclonal Antibody, Cy7 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat, Cynomolgus Monkey
Predicted Reactivity Human, Cow, Pig
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PSME1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5720
Swiss Prot Q06323
Subcellular location Cytoplasm
Synonyms 11S regulator complex alpha subunit; 11S regulator complex subunit alpha; 29 kD MCP activator subunit; 29kD MCP activator subunit; Activator of multicatalytic protease subunit 1; IFI5111; IGUP I-5111; Interferon gamma IEF SSP 5111; Interferon gamma inducible protein 5111; Interferon gamma up regulated I 5111 protein; Interferon gamma up-regulated I-5111 protein; MGC8628; PA28a; PA28alpha; Proteasome (prosome, macropain) activator subunit 1 (PA28 alpha); Proteasome activator 28 subunit alpha; Proteasome activator complex subunit 1; Proteasome activator subunit 1; PSME1; PSME1_HUMAN; REG-alpha; REGalpha
Background Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is PSME1 Polyclonal Antibody, Cy7 Conjugated (bs-19575R-Cy7) validated for, and at what dilution?

bs-19575R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PSME1 Polyclonal Antibody, Cy7 Conjugated (bs-19575R-Cy7) react with?

Tested reactivity: Mouse, Rat, Cynomolgus Monkey. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Pig.

How should bs-19575R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PSME1 Polyclonal Antibody, Cy7 Conjugated (bs-19575R-Cy7)?

KLH conjugated synthetic peptide derived from human PSME1

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-19575R-Cy7)?

Cy7 on bs-19575R-Cy7: excitation / emission 743nm/767nm.