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SEPHS1 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-19627R-Cy3
Product Name SEPHS1 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SEPHS1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 22929
Swiss Prot P49903
Subcellular location Cytoplasm, Cell membrane
Synonyms MGC4980; SELD; SPS1_HUMAN; Selenide, water dikinase 1; Selenium donor protein 1; Selenophosphate synthetase 1; SEPHS1; SPS; SPS1
Background Synthesizes selenophosphate from selenide and ATP.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is SEPHS1 Polyclonal Antibody, Cy3 Conjugated (bs-19627R-Cy3) validated for, and at what dilution?

bs-19627R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SEPHS1 Polyclonal Antibody, Cy3 Conjugated (bs-19627R-Cy3) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit.

How should bs-19627R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SEPHS1 Polyclonal Antibody, Cy3 Conjugated (bs-19627R-Cy3)?

KLH conjugated synthetic peptide derived from human SEPHS1

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-19627R-Cy3)?

Cy3 on bs-19627R-Cy3: excitation / emission 512,550nm/570,615nm.