OMA1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-19641R-Cy3 |
| Product Name | OMA1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Pig, Horse, Chicken, Cat, Rabbit, Zebrafish |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human OMA1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 115209 |
| Swiss Prot | Q96E52 |
| Subcellular location | Cytoplasm |
| Synonyms | Metalloendopeptidase OMA1, mitochondrial; OMA1; Metalloprotease-related protein 1; MPRP-1; Overlapping with the m-AAA protease 1 homolog; OMA1; MPRP1 |
| Background | Metalloprotease that is part of the quality control system in the inner membrane of mitochondria. Following stress conditions that induce loss of mitochondrial membrane potential, mediates cleavage of OPA1 at S1 position, leading to OPA1 inactivation and negative regulation of mitochondrial fusion. May also cleave UQCC3 under these conditions. Its role in mitochondrial quality control is essential for regulating lipid metabolism as well as to maintain body temperature and energy expenditure under cold-stress conditions. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is OMA1 Polyclonal Antibody, Cy3 Conjugated (bs-19641R-Cy3) validated for, and at what dilution?
bs-19641R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does OMA1 Polyclonal Antibody, Cy3 Conjugated (bs-19641R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig, Horse, Chicken, Cat, Rabbit, Zebrafish.
How should bs-19641R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise OMA1 Polyclonal Antibody, Cy3 Conjugated (bs-19641R-Cy3)?
KLH conjugated synthetic peptide derived from human OMA1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-19641R-Cy3)?
Cy3 on bs-19641R-Cy3: excitation / emission 512,550nm/570,615nm.