PURA Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-19685R-BF750-100uL |
| Product Name | PURA Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Zebrafish |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Cat, Rabbit, Guinea Pig |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PURA |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5813 |
| Swiss Prot | Q00577 |
| Subcellular location | Nucleus |
| Synonyms | PUR ALPHA; PUR1; purA; PURA_HUMAN; PURALPHA; Purine rich element binding protein A; Purine rich single stranded DNA binding protein alpha; Purine-rich single-stranded DNA-binding protein alpha; Transcriptional activator protein PUR alpha; Transcriptional activator protein Pur-alpha. |
| Background | This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PURA Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-19685R-BF750) validated for, and at what dilution?
bs-19685R-BF750 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PURA Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-19685R-BF750) react with?
Tested reactivity: Mouse, Zebrafish. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Cat, Rabbit, Guinea Pig.
How should bs-19685R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PURA Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-19685R-BF750)?
KLH conjugated synthetic peptide derived from human PURA
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-19685R-BF750)?
AbBy Fluor-750 on bs-19685R-BF750: excitation / emission 749nm/775nm.