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PURA Polyclonal Antibody, Biotin Conjugated

Applications

  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Mouse
  • Zebrafish

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Cat
  • Rabbit
  • Guinea Pig
Overview
Catalog # bs-19685R-Biotin
Product Name PURA Polyclonal Antibody, Biotin Conjugated
Applications
ELISA IHC-P IHC-F
Reactivity Mouse, Zebrafish
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Pig, Cat, Rabbit, Guinea Pig
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PURA
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 5813
Swiss Prot Q00577
Subcellular location Nucleus
Synonyms PUR ALPHA; PUR1; purA; PURA_HUMAN; PURALPHA; Purine rich element binding protein A; Purine rich single stranded DNA binding protein alpha; Purine-rich single-stranded DNA-binding protein alpha; Transcriptional activator protein PUR alpha; Transcriptional activator protein Pur-alpha.
Background This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008]
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is PURA Polyclonal Antibody, Biotin Conjugated (bs-19685R-Biotin) validated for, and at what dilution?

bs-19685R-Biotin is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PURA Polyclonal Antibody, Biotin Conjugated (bs-19685R-Biotin) react with?

Tested reactivity: Mouse, Zebrafish. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Cat, Rabbit, Guinea Pig.

How should bs-19685R-Biotin be stored?

Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PURA Polyclonal Antibody, Biotin Conjugated (bs-19685R-Biotin)?

KLH conjugated synthetic peptide derived from human PURA